vIL10 does not affect the quantity of latent virus. Spleen cells underwent three-fold dilutions with 12 duplicate wells per dilution. Viral DNA was detected by nested PCR to orf50. Curve-fit lines represent results of nonlinear regression analysis. Data points represent the mean of two independent experiments with spleens pooled from five mice per experiment. Results indicate no difference between MHV-76 and recombinants with or without vIL10.