vIL10 increases acute-phase titer in lungs. Four- to six-week-old BALB/c mice were infected i.n. with 2x105 pfu of virus in 40 milliliters. Viral titers from homogenized left lungs were determined on NIH-3T3 cells. Data represent three independent experiments with titers performed in duplicate. Pairwise statistical analysis shows no significant differences between titers of MHV68 or 76 or between either of these viruses and the recombinants. Statistical significance by unpaired t-test with Welch’s correction is indicated (*): 76.vIL10 vs MHV-76 (P = 0.0025), 76.vIL10 vs 76.rev (P < 0.0001), MHV-68 vs 76.rev (P = 0.0076).