vIL-10 is expressed in transfected cells. NIH-3T3 cells were transfected with plasmid containing the Pgp150.vIL10 expression cassette or control plasmid (pGL3-Promoter) and infected or not with MHV-76 at a MOI of 1. After 24, 36, and 48 hours, supernatant was tested for the presence of vIL-10 by ELISA. Data points represent the averages of two readings of each sample in duplicate experiments. Error bars indicate +/- 1 standard deviation.