Human cytomegalovirus induces apoptosis in neural stem/progenitor cells derived from induced pluripotent stem cells by generating mitochondrial dysfunction and endoplasmic reticulum stress

Table 1 List of primer sequences for RT-PCR

Gene	Forward primer	Reverse primer
IE1*	ATGGAGTCCTCTGCCAAGAG	ATTCTATGCCGCACCATGTCC
IE2*	ATGGAGTCCTCTGCCAAGAG	CTGAGACTTGTTCCTCAGGTCCTG
vIL-10*	ATGCTGTCGGTGATGGTCTCTTCC	CTTTCTCGAGTGCAGATACTCTTCG
UL36*	GACCTACGGGACACGCTGATG	TGTGGAAGTGGTCGCAGTGAC
UL38	GACTACGACCACGCATAGCA	GGGAACAGAGCGTTCCAATA
pp65	CGCAACCTGGTGCCCATGG	CGTTTGGGTTGCGCAGCGGG
Nanog*	GCTTGCCTTGCTTTGAAGCA	TTCTTGACCGGGACCTTGTC
Oct-4	GAGCAAAACCCGGAGGAGT	TTCTCTTTCGGGCCTGCAC
Sox1	GCGGAAAGCGTTTTCTTTG	TAATCTGACTTCTCCTCCC
Sox2	ATGCACCGCTACGACGTGA	CTTTTGCACCCCTCCCATTT
Pax6*	AACAGACACAGCCCTCACAAACA	CGGGAACTTGAACTGGAACTGAC
Nestin*	CAGCGTTGGAACAGAGGTTGG	TGGCACAGGTGTCTCAAGGGTAG
MAP2*	CCACCTGAGATTAAGGATCA	GGCTTACTTTGCTTCTCTGA
GFAP*	GTACCAGGACCTGCTCAAT	CAACTATCCTGCTTCTGCTC
OSP*	ACTGCTGCTGACTGTTCTTC	GTAGAAACGGTTTTCACCAA
XBP1*	CCTTGTAGTTGAGAACCAGG	GGGGCTTGGTATATATGTGG
CHOP*	TGGAAGCCTGGTATGAGGAC	TCACCATTCGGTCAATCAGA
β-actin*	ACCATGGATGATGATATCGC	TCATTGTAGAAGGTGTGGTG
GAPDH*	CCACCCATGGCAAATTCCATGGCA	TCTAGACGGCAGGTCAGGTCCACC

Asterisks (*) indicate that amplified fragments contain splicing junctions. Amplified fragments for UL38, pp65, Oct-4, Sox1, and Sox2 did not contain splicing junctions, and therefore control experiments without reverse transcriptase confirmed the RNA origin of the PCR products.