|
Gene
|
Forward primer
|
Reverse primer
|
|---|
|
IE1*
|
ATGGAGTCCTCTGCCAAGAG
|
ATTCTATGCCGCACCATGTCC
|
|
IE2*
|
ATGGAGTCCTCTGCCAAGAG
|
CTGAGACTTGTTCCTCAGGTCCTG
|
|
vIL-10*
|
ATGCTGTCGGTGATGGTCTCTTCC
|
CTTTCTCGAGTGCAGATACTCTTCG
|
|
UL36*
|
GACCTACGGGACACGCTGATG
|
TGTGGAAGTGGTCGCAGTGAC
|
|
UL38
|
GACTACGACCACGCATAGCA
|
GGGAACAGAGCGTTCCAATA
|
|
pp65
|
CGCAACCTGGTGCCCATGG
|
CGTTTGGGTTGCGCAGCGGG
|
|
Nanog*
|
GCTTGCCTTGCTTTGAAGCA
|
TTCTTGACCGGGACCTTGTC
|
|
Oct-4
|
GAGCAAAACCCGGAGGAGT
|
TTCTCTTTCGGGCCTGCAC
|
|
Sox1
|
GCGGAAAGCGTTTTCTTTG
|
TAATCTGACTTCTCCTCCC
|
|
Sox2
|
ATGCACCGCTACGACGTGA
|
CTTTTGCACCCCTCCCATTT
|
|
Pax6*
|
AACAGACACAGCCCTCACAAACA
|
CGGGAACTTGAACTGGAACTGAC
|
|
Nestin*
|
CAGCGTTGGAACAGAGGTTGG
|
TGGCACAGGTGTCTCAAGGGTAG
|
|
MAP2*
|
CCACCTGAGATTAAGGATCA
|
GGCTTACTTTGCTTCTCTGA
|
|
GFAP*
|
GTACCAGGACCTGCTCAAT
|
CAACTATCCTGCTTCTGCTC
|
|
OSP*
|
ACTGCTGCTGACTGTTCTTC
|
GTAGAAACGGTTTTCACCAA
|
|
XBP1*
|
CCTTGTAGTTGAGAACCAGG
|
GGGGCTTGGTATATATGTGG
|
|
CHOP*
|
TGGAAGCCTGGTATGAGGAC
|
TCACCATTCGGTCAATCAGA
|
|
β-actin*
|
ACCATGGATGATGATATCGC
|
TCATTGTAGAAGGTGTGGTG
|
|
GAPDH*
|
CCACCCATGGCAAATTCCATGGCA
|
TCTAGACGGCAGGTCAGGTCCACC
|
- Asterisks (*) indicate that amplified fragments contain splicing junctions. Amplified fragments for UL38, pp65, Oct-4, Sox1, and Sox2 did not contain splicing junctions, and therefore control experiments without reverse transcriptase confirmed the RNA origin of the PCR products.
