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Figure 3 | Herpesviridae

Figure 3

From: Human cytomegalovirus induces apoptosis in neural stem/progenitor cells derived from induced pluripotent stem cells by generating mitochondrial dysfunction and endoplasmic reticulum stress

Figure 3

HCMV-induced apoptosis of NSPC/iPSCs. (A) Towne-infected NSPC/iPSCs at 6 dpi were subjected to TUNEL assay followed by immunofluorescence test with an anti-gB antibody. TUNEL-positive nuclei were stained in red. The anti-gB antibody was detected with Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (green). Nuclei were stained with DAPI. (B-D) Towne-infected NSPC/iPSCs at 3 dpi were subjected to immunofluorescence test with anti-IE1/IE2 antibody in combination with anti-cleaved caspase 3 (B), anti-cleaved caspase 9 (C), or anti-cytochrome c (D) antibody. Alexa Fluor 488-conjugated goat anti-mouse IgG (green) or Alexa Fluor 594-conjugated goat anti-rabbit IgG antibody (red) was used as a secondary antibody. Nuclei were stained with DAPI. (E) Mock-infected NSPC/iPSCs were subjected to immunofluorescence test with anti-IE1/IE2 antibody in combination with anti-cleaved caspase 3 (upper panel), anti-cleaved caspase 9 (middle panel), or anti-cytochrome c (lower panel) antibody. Nuclei were stained with DAPI. Representative results from two independent experiments are shown.

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