Spatial distribution of FN on days 6 and 12 of culture. A-D. NB + 6. E-H. NB + 12. Control NB + 6 (A) and NB + 12 (E) SMGs show a distinct and well-demarcated pattern of FN immunoreactivity at the BMZ (white arrows). In mCMV-infected SMGs, there is a marked change in FN localization in both NB + 6 (B) and NB + 12 (F) glands. FN immunostaining surrounds individual metaplastic stromal cells (white arrowheads), with a notable decline in FN immunoreactivity at the BMZ being seen (double white arrows). In GEF-treated, mCMV-infected NB + 6 SMGs (C), FN immunoreactivity is seen surrounding a small number of abnormal stromal cells (white arrowheads) and is mostly absent from BMZ (double white arrows). In U0126-treated, mCMV-infected SMGs (D), FN is immunolocalized at the BMZ (white arrow) in a pattern closely resembling that seen in controls (compare D to A) and markedly different from that seen in untreated, mCMV-infected SMGs (compare D to B). In contrast, the distribution of FN immunolocalization in GEF-treated (G) and U0126-treated (H), mCMV-infected NB + 12 SMGs resembles that seen in untreated mCMV-infected SMGs (compare G, H to F); FN immunoreactivity shows a pericellular distribution on stromal cells (white arrowheads) and near absence at the BMZ (double white arrows). Bar: A-B, D-H- 37 μm, C- 45 μm.