mCMV-induced changes in the spatial distribution of IL-6 on days 6 and 12 of culture. A-D. NB + 6 SMGs. E-H. NB + 12 SMGs. In controls (A, E), IL-6 immunostain is weakly seen on ductal and proacinar epithelia (e); it is also localized in nerves (arrows). With mCMV infection (B, F), there is a marked increase in immunodetectable IL-6 in abnormal stromal cells (arrowheads) but is relative absent from epithelia. Insets: Higher magnification of IL-6 stained stromal cells. In GEF-treated, mCMV-infected NB + 6 SMGs (C), IL-6 immunoreactivity is seen primarily on stromal cells (arrowheads). In U0126-treated, mCMV-infected NB + 6 SMGs (D), IL-6 immunostaining resembles that seen in controls; IL-6 is weakly seen on epithelia (e) but absent from stroma (s). On day 12, GEF-treated, mCMV-infected (G) or U0126, mCMV-infected (H) SMGs exhibit IL-6 immunoreactivity throughout the stromal cells (black arrowheads) and absence from epithelia. Bar: 50μm; Insets B, F -30 μm.