Figure 3

Chemokine mRNA accumulation during HSV-1 infection via IFI16-dependent and IFI16-independent mechanism. Macrophages were transfected with siRNA targeting IFI16 or control siRNA for 48 hours before infection with HSV-1 (KOS, MOI of 1) or stimulation with IFN-α (1000 U/ml). Mock infections were made as control. A) After 6 h, total RNA was harvested and IFI16 mRNA-levels were assayed using RT-qPCR. B) IFI16 protein-levels were evaluated by Western blotting in untreated macrophages 48 hours after transfection with control siRNA and IFI16 siRNA. C and D) Total RNA harvested at 6 hours post infection was analyzed for expression of CCL3 and CXCL10 using RT-qPCR. Results depicted represent one of four donors (A, C and D) and three donors (B) with similar results. *, p<0.05 and **, p<0.01 (t-test).