IFI16 expression in human macrophages. A) Macrophages were stimulated with IFN-α (1000 U/ml) for 6 hours and intracellular levels of IFI16 were assessed by Western blotting. B and C) Macrophages were stimulated with R848 (1 μg/ml) or infected with HSV-1 or UV-inactivated HSV-1 (KOS, MOI of 1) for 6 hours before assessing IFI16 mRNA-levels by RT-qPCR. The Western blot shown in A (lower panel) represents one of three donors showing similar results and the quantified data (upper panel) represents the mean +/−SD of the three donors from one experiment. The data depicted in B and C represents mean +/− SD of at least two donors of one of two independent experiments with similar results. *, p<0.05 (t-test).