Figure 6
HSV-1 DNA positive cells remain in an area of demyelination following the clearance of viral antigen. 35S-labelled HSV-1 DNA positive cells can remain in demyelinating lesions of 10-12 week ♀SJL/J, A/J, and PL/J mice infected with virus via the oral mucosa. Adjacent serial sections were examined by ISH and histology. In representative sections from the CB of HSV-1 infected PL/J mice (a) demyelinating lesion are identified, 26 days PI, (x100), LFB-CFV while adjacent sections identify (b) HSV-1 DNA positive cells (arrow), 26 days PI, (x200), ISH counterstained with H & E. (c) Higher power photo-micrograph of HSV-1 DNA positive cells (arrow), 26 days PI, (x400), ISH/H & E. (d) High power photo-micrograph of HSV-1 DNA positive cell, 26 days PI (x1000), ISH/H & E.