Figure 3

'Focal' areas of HSV-1 DNA and antigen positive cells develop sequentially in non-contiguous areas of the BST and CB of PL/J mice. Brains from 10-12 week ♀ PL/J mice, infected with HSV-1 via the oral mucosa, were examined for viral DNA and antigen. Serial sections of the brain were examined for ³⁵S-labelled HSV DNA (ISH) and viral antigens (IHC) and counterstained with either H & E or CFV respectively. 'Focal' areas of ³⁵S-labelled HSV-1 DNA (Figure 3 a-c) and viral antigen (Figure 3 d-f) positive cells are identified in the BST (a) A 'focal' area of HSV-1 DNA positive cells in the BST (arrow), 3 days PI (x100) (b) Higher power photomicrograph, 3 days PI (x 200) (c) High power photomicrograph, 3 days PI (x400) (d) A 'focal' area of viral antigen positive cells in the BST (arrow), 5 days PI (x100) (e) Higher power photomicrograph, 5 days PI (x400) (f) Higher power photomicrograph of viral antigen positive neuron, 5 days PI (x400). 'Focal' areas of ³⁵S-labelled HSV-1 DNA (Figure 3g-i) and viral antigen (Figure 3 j-l) positive cells are identified in the CB. (g) A 'focal' area of HSV-1 DNA positive cells in the CB (arrow), 5 days PI (x100) (h) Higher power photomicrograph, 5 days PI (x200) (i) High power photomicrograph, 5 days PI (x400) (j) A 'focal' area of viral antigen positive cells in the CB, 6 days PI (x200) (k) Higher power photomicrograph, 6 days PI (x400) (l) Higher power photomicrograph of viral antigen positive Purkinje cells, 6 days PI (x400).