Figure 2

HSV-1 antigen positive cells appear sequentially in non-contiguous areas throughout the brains of BALB/c mice. Ten-12 week ♀ BALB/c mice were infected with HSV-1 strain 2 via the oral mucosa. Three mice were examined every 3 days at necropsy from day 0 through day 30 PI. Serial sections of the brain were examined for viral antigens (IHC) and counterstained with CFV. In representative sections, viral antigen was first identified in the BST on day 3 PI. (a) Viral antigen positive cells in the BST, 3 days PI (x100) (b) Higher power photomicrograph of HSV-1 antigen positive cells on day 3 PI (x400) (c) High power photomicrograph of HSV-1 antigen positive cell with the morphology of a neuron on day 3 PI (x1000). Viral antigen was first identified in the cerebellum (CB) on day 6 PI (d) Viral antigen positive cells in the CB, 6 days PI (x100) (e) Higher power photomicrograph of HSV-1 antigen positive cells on day 6 PI (x400) (f) High power photomicrograph of HSV-1 antigen positive Purkinje cells in the CB, 6 days PI (x400). Viral antigen was first identified in the cerebrum (CR) on day 7 PI (g) Viral antigen positive cells (arrow) in the CR, 7 days PI (x200) (h) Higher power photomicrograph of HSV-1 antigen positive cells with the morphology of neurons in the CR, 7 days PI (X400).