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Figure 1 | Herpesviridae

Figure 1

From: The effect of mouse strain on herpes simplex virus type 1 (HSV-1) infection of the central nervous system (CNS)

Figure 1

HSV-1 DNA and antigen positive cells are not present diffusely throughout the BST of BL/6 mice but are restricted to an area associated with the RTNBST. Ten-12 week ♀ BL/6 mice were infected with HSV-1 strain 2 via the oral mucosa. Three mice were examined every 3 days at necropsy from day 0 through 30 PI. Six micron thick serial sections of the brain were examined for 35S-labelled HSV DNA (ISH) and viral antigens (IHC) and counterstained with either H & E or CFV respectively. In representative sections, both viral DNA (Figure 1 a - c) and viral antigen (Figure 1 d - f) are identified on day 3 PI and restricted to a small area of the BST associated with the roots of the trigeminal nerve (RTNBST). (a) Small area of 35S-labelled HSV DNA positive cells (arrow) in the BST on day 3 PI (x100) (b) Higher power photomicrograph of HSV-1 DNA positive cells (arrow) on day 3 PI (x200) (c) High power photomicrograph of HSV-1 DNA positive cells on day 3 PI (x400) (d) Small area of HSV-1 antigen positive cells in the BST on day 3 PI (x100) (e) Higher power photomicrograph of HSV-1 antigen positive cells on day 3 PI (x200) (f) High power photomicrograph of HSV-1 antigen positive cells on day 3 PI (x400).

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