Effect of shRNAs 1 and 2 on MCMV replication at m.o.i. = 0.2. a) An SEC expressing shRNA-2 was transfected into M2-10B4 cells 24 hrs prior to infection with MCMV at an m.o.i. of 0.2. b) Western blot of IE-3 protein levels in shRNA-2 treated and control shRNA treated samples Effect of MCMV specific shRNA at low m.o.i.'s in vitro. c) Virus titers at day 5 p.i. in M2-10B4 cultures infected with MCMV at the indicated m.o.i.'s. Dark bars: 50 nM IE-3 specific shRNA-1, white bars: control shRNA. d) Western blot showing the time course of IE-3 protein expression in MCMV-infected M2-10B4 cells (m.o.i. = 0.2) following transfection of the IE-3 shRNA-1 expressing plasmid. e) Viral titers at day 5 p.i. in M2-10B4 cultures infected with MCMV at various m.o.i.'s and treated with either shRNA-1a or shRNA-2a.