Detection of human cytomegalovirus in motile spermatozoa and spermatogenic cells in testis organotypic culture

Herpesviridae

Table 4 Quantitative analysis of immature and mature germ cells in HCMV-infected human testis organotypic culture

Germ cells	Organotypic culture in vitro, days
	7			14
	Uninfected	HCMV-infected	P-value	Uninfected	HCMV-infected	P-value
Spermatogonia	100^a	53,8	<0.001^b	82,2	36,8	<0.001
Spermatocytes	100	38,9	<0.001	51,5	18,7	<0.001
Round spermatids	104	37,7	<0.001	70,4	27,6	<0.001
Elongated spermatids	96	57,7	<0.001	65,7	42,5	<0.05
Spermatozoa	106,3	100	>0.05	106,3	100	>0.05

^a - mean quantity of cells, %; quantity of cells before introduction in culture was taken for 100%;
^b - Student's paired t-test.