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Figure 5 | Herpesviridae

Figure 5

From: Circulating herpes simplex type 1 (HSV-1)-specific CD8+T cells do not access HSV-1 latently infected trigeminal ganglia

Figure 5

Following disruption of the TG-resident CD8+ T cell population via stress and corticosterone treatment, in vitro generated effector or memory cells do not enter the TG. Spleens from naïve gBT I.1 Thy 1.1 mice were pulsed with SSIEFARL (gB498-605) and incubated in IL-2 for three days (effector cells) and some cells were further incubated for 10 days with IL-15 replacing IL-2 to generate memory cells. (A) Cells were stained for CD44, CD69, LFA-1, and VLA-4 and analysed by flow cytometry. Latently infected wild type mice (Thy1.2+) were subjected to 4 consecutive days of restraint stress and corticosterone. After the final treatment mice received an adoptive transfer of 106 effector (B) or memory (C) CD8+ T cells (Thy1.1+). Four days after the adoptive transfer, spleens, blood, lungs, and TGs were harvested and stained for CD8¿, CD45, Thy1.1 (Donor), Thy1.2 (Recipient), and gB-specific T cell receptor. Data represent means and standard errors of the percentages of gB-CD8 that are of donor (Thy1.1) or recipient (Thy1.2) origin in the specified tissues (n = 4 mice per group). The experiments were repeated with similar results.

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