Figure 1

Effect of systemic TAK-779 treatment on CD8⁺ T cell infiltration into HSV-1-infected ganglia. Six days following corneal HSV-1 infection, B6 mice were treated subcutaneously with 150 ¿g/mouse TAK-779 or with an equal volume of PBS. Two days following treatment, TG were excised and dispersed into single cells. The cells were stained for surface CD8¿, CD45, and gB-specific T cell receptor, and analyzed by flow cytometry. Data represent the mean ± standard error of cumulative data for total CD8⁺ T cells (A) or gB-CD8 (B) per TG from three experiments involving a total of 6 PBS-treated and 11 TAK-779 treated mice. (C) DNA extracted from dispersed TG cells and analyzed by real-time PCR for the number of viral glycoprotein H (gH) gene copies revealed no significant group differences. A two-tailed Students t test was used for all statistical analyses.