Figure 8
Recognition of Hodgkin's lymphoma cells by LMP2 345-352 specific T cell clone #3. A: Substitution of position 3 in the cognate LMP2345-352 peptide with the favored HLA-B8 anchor amino acid K leads to abolishment of the recognition by LMP2345-352 specific CD8+ T cell clones as exemplified by clone #3. The indicated peptides were added to the clonal T cells. B: HLA-B8+ EBV- Hodgkin's lymphoma cells were either left unmanipulated, peptide pulsed, infected with recombinant vaccinia virus encoding LMP2 (vvLMP2) or vaccinia control virus (vvTK-) for stimulation of the LMP2345-352 specific CD8+ T cell clone #3 in IFNγ ELISPOT assays. Data for one representative of five LMP2345-352 specific CD8+ T cell clones are shown. 105 clonal T cells were added per ELISPOT well.