Peptide affinity and HLA restriction by LMP2
specific T cell clone #3. IFN-γ ELISPOT assays were performed with HLA-B8+ SO-LCL and BM-LCL as well as HLA-B8- LG2 and T2 cell lines as stimulators for the LMP2 specific T cell clone #3 in the presence of titrated doses of the αHLA class I antibody w6/32, the αHLA-DR antibody L243 and the αHLA-B8 antibody (A). Peptide affinity of clone #3 was determined by direct addition of increasing concentrations of the LMP2345-352 and the LMP2347-355 peptides to clonal T cells in INFγ ELISPOT assays (B). Data for one representative of five LMP2345-352 specific CD8+ T cell clones are shown. 105 clonal T cells were added per ELISPOT well.