CD69 up-regulation and IFN-γ secretion by LMP2 and EBNA3A specific HLA-B8 restricted CD8+T cell clones. Clonal T cells were stimulated with medium, PHA, the control LMP2347-355 peptide or the cognate LMP2345-352 or EBNA3A325-333 peptides. CD69 surface expression and intracellular IFN-γ was analyzed. Dot blot and histogram analysis was performed after gating on CD8+ T cells. The LMP2345-352 specific CD8+ T cell clone #3 (A) and the EBNA3A325-333 specific CD8+ T cell clone MS.B11 (B) were analyzed. The presented data are representative for five LMP2345-352 specific CD8+ T cell clones.