Comparison of LMP2
frequency with EBNA3A
in HLA-B8+/-A2+and HLA-B8+/-A2- donors after in vitro stimulation. IFN-γ ELISPOT assays were performed with CD8+ T cell cultures expanded for 1 week with peptide pulsed DCs (stimulatory peptides are indicated above panels) and restimulation for IFN-γ secretion by direct addition of 10 μM peptide (readout peptides are indicated in the legend). In HLA-A2+/-B8+ donors, the LMP2426-434 and LMP2345-352 specific responses were compared (A). In HLA-B8+/-A2- donors, the EBNA3A325-333 and LMP2345-352 specific responses were addressed (B). One representative of three donors is shown in (A) and in (B). 105 and 104 expanded T cells were added per ELISPOT well in (A) and (B), respectively.