Figure 3

Comparison of LMP2 _345-352 frequency with EBNA3A _325-333 and LMP2 _426-434 in HLA-B8⁺/-A2⁺and HLA-B8⁺/-A2^- donors after in vitro stimulation. IFN-γ ELISPOT assays were performed with CD8⁺ T cell cultures expanded for 1 week with peptide pulsed DCs (stimulatory peptides are indicated above panels) and restimulation for IFN-γ secretion by direct addition of 10 μM peptide (readout peptides are indicated in the legend). In HLA-A2⁺/-B8⁺ donors, the LMP2_426-434 and LMP2_345-352 specific responses were compared (A). In HLA-B8⁺/-A2^- donors, the EBNA3A_325-333 and LMP2_345-352 specific responses were addressed (B). One representative of three donors is shown in (A) and in (B). 10⁵ and 10⁴ expanded T cells were added per ELISPOT well in (A) and (B), respectively.