Frequency of LMP2
specific CD8+T cells in whole blood. Whole blood of three representative of six HLA-B8 positive donors was stimulated with medium, PHA or the EBNA3A325-333, LMP2345-352, LMP2347-355 peptides. CD69 up-regulation and intracellular IFN-γ production was measured by flow cytometry after gating on living CD8+ T cells. The indicated percentages represent CD69+/IFN-γ+ (upper number) and CD69+/INF-γ- (lower number) populations.