Figure 5
RCMV increases expression of MCP-1 in the aortic allografts and cells from the infected adventitia migrate towards the intima. (A) Cross sections of the RCMV-infected and uninfected allografts (controls) at 2 and 8 weeks post transplantation stained for MCP-1. Green, MCP-1. Bar chart shows MCP-1 expression in the allografts, presented as positive area of the cross section (μm2). Both at 2 and 8 weeks after transplantation MCP-1 expression was increased in infected allografts (7-fold at 2 weeks and 3.5-fold at 8 weeks post transplantation). ***p < 0.001 vs.uninfected control at 2 and 8 weeks, respectively. I, intima; M, media; A, adventitia. (B) Cross section of the aortic allograft with transplanted fluorescently labeled isogenic adventitial graft. Grafts were collected 2 weeks post adventitia transplantation. Picture shows labeled blue adventitial cells in the allograft intima. Green, SM-actin; Blue, migrated adventitial cells; Red, nuclei.