Figure 1

The EBV BAC system: an overview. The cloned EBV-BAC can be manipulated in E.coli cells using multiple techniques that rely on homologous recombination. The mutated EBV BAC is then introduced into 293 cells and selected with an antibiotic to create a producer cell line from which infectious particles that contain the mutant EBV BAC can be produced. The episomes present in the producer cell line can be extracted and reintroduced in E.coli where multiple controls such as restriction analyses, Southern blotting and sequencing, can be readily performed.