Chromosomal integration of an avian oncogenic herpesvirus reveals telomeric preferences and evidence for lymphoma clonality

Table 2 A cytogenomic profile of lymphomas from MDV-infected birds: virus integrations, ploidy, and telomeres.

Sample Information			MDV Integration Sites*			Ploidy Levels (metaphase cells)^†		Mega-Telomere Signals^‡
Bird #	Sex	Tumor # (Tissue Source)	Mode	Mean	SD	% Tetraploid	% Aneuploid	Mode	W
1	M	1-T1 (heart)	5	4.4	0.9	2	0	7	-
		1-T2 (left gonad)	5	5.4	1.3	0	0	11	-
		1-T3 (lung)	5	4.9	0.7	0	0	9	-
2	M	2-T1 (left gonad)	4	3.8	0.7	4	0	7	-
		2-T2 (right gonad)	4	4.1	1.1	4.5	0	7	-
3	M	3-T1 (lung)	4	4.7	1.6	4	0	6	-
		3-T2 (bursa)	5	4.3	1.3	16	0	7	-
		3-T3 (gonad)	6	5.9	0.8	5	0	6	-
4	F	4-T1 (spleen 1)	3	3.2	1.2	0	0	9	Yes
		4-T2 (spleen 2)	2	1.9	1.2	0	0	7	Yes
		4-T3 (heart)	1	1.4	1.3	0	0	7	Yes
5	M	5-T1 (left gonad)	4	3.8	0.8	0	0	8	-
		5-T2 (right gonad)	4	3.6	0.8	6	0	8	-
6	M	6-T1 (heart)	2	1.6	1.2	0	0	7	-
7	F	7-T1 (spleen)	0	0.2	0.6	0	0	8	Yes
8	F	8-T1 (gonad)	3	2.4	1.4	0	0	10	Yes
9	F	9-T1 (liver)	1	0.5	0.5	0	0	8	Yes
10^§	F	10-T1 (gonad)	5	5.0	1.0	25	12.5	9	Yes
11^§	M	11-T1 (kidney)	3	3.5	2.2	21	23	5	-
		11-T2 (liver)	6	4.5	2.2	17.5	15	7	-
		11-T3 (thymus)	6	5.4	1.3	20	2.5	8	-
12^§	F	12-T1 (gonad)	5	5.6	2.0	27	7	6	Yes
		12-T2 (kidney)	7	5.5	2.2	0	0	6	-^¶
13^§	F	13-T1 (spleen)	9	7.2	3.0	2.5	0	7	Yes
		13-T2 (liver)	9	8.0	1.8	0	10	7	Yes
		13-T3 (kidney)	9	7.7	2.1	10	7	7	Yes

*Mode (most frequent value), mean, and standard deviation (SD) of diploid metaphase cells.
^†Tetraploid cells were identified as those containing four copies of all macrochromosomes and sex chromosomes, and an estimated number of microchromosomes present in twice the normal amount. Aneuploid cells were identified as those containing an abnormal copy number (less than or greater than two) of one or more macrochromosomes.
^‡Mega-telomere signals were counted in tumor metaphase cells using the telomere-PNA probe, and the mode was calculated for each tumor.
^§Birds from infection strategy 2 (see Methods section for details).
^¶Tumor 12-T2 lacked a W chromosome (as verified by the W BAC probe, see Table 1).