|
Sample Information
|
MDV Integration Sites*
|
Ploidy Levels (metaphase cells)†
|
Mega-Telomere Signals‡
|
|---|
|
Bird #
|
Sex
|
Tumor # (Tissue Source)
|
Mode
|
Mean
|
SD
|
% Tetraploid
|
% Aneuploid
|
Mode
|
W
|
|---|
|
1
|
M
|
1-T1 (heart)
|
5
|
4.4
|
0.9
|
2
|
0
|
7
|
-
|
| | |
1-T2 (left gonad)
|
5
|
5.4
|
1.3
|
0
|
0
|
11
|
-
|
| | |
1-T3 (lung)
|
5
|
4.9
|
0.7
|
0
|
0
|
9
|
-
|
|
2
|
M
|
2-T1 (left gonad)
|
4
|
3.8
|
0.7
|
4
|
0
|
7
|
-
|
| | |
2-T2 (right gonad)
|
4
|
4.1
|
1.1
|
4.5
|
0
|
7
|
-
|
|
3
|
M
|
3-T1 (lung)
|
4
|
4.7
|
1.6
|
4
|
0
|
6
|
-
|
| | |
3-T2 (bursa)
|
5
|
4.3
|
1.3
|
16
|
0
|
7
|
-
|
| | |
3-T3 (gonad)
|
6
|
5.9
|
0.8
|
5
|
0
|
6
|
-
|
|
4
|
F
|
4-T1 (spleen 1)
|
3
|
3.2
|
1.2
|
0
|
0
|
9
|
Yes
|
| | |
4-T2 (spleen 2)
|
2
|
1.9
|
1.2
|
0
|
0
|
7
|
Yes
|
| | |
4-T3 (heart)
|
1
|
1.4
|
1.3
|
0
|
0
|
7
|
Yes
|
|
5
|
M
|
5-T1 (left gonad)
|
4
|
3.8
|
0.8
|
0
|
0
|
8
|
-
|
| | |
5-T2 (right gonad)
|
4
|
3.6
|
0.8
|
6
|
0
|
8
|
-
|
|
6
|
M
|
6-T1 (heart)
|
2
|
1.6
|
1.2
|
0
|
0
|
7
|
-
|
|
7
|
F
|
7-T1 (spleen)
|
0
|
0.2
|
0.6
|
0
|
0
|
8
|
Yes
|
|
8
|
F
|
8-T1 (gonad)
|
3
|
2.4
|
1.4
|
0
|
0
|
10
|
Yes
|
|
9
|
F
|
9-T1 (liver)
|
1
|
0.5
|
0.5
|
0
|
0
|
8
|
Yes
|
|
10§
|
F
|
10-T1 (gonad)
|
5
|
5.0
|
1.0
|
25
|
12.5
|
9
|
Yes
|
|
11§
|
M
|
11-T1 (kidney)
|
3
|
3.5
|
2.2
|
21
|
23
|
5
|
-
|
| | |
11-T2 (liver)
|
6
|
4.5
|
2.2
|
17.5
|
15
|
7
|
-
|
| | |
11-T3 (thymus)
|
6
|
5.4
|
1.3
|
20
|
2.5
|
8
|
-
|
|
12§
|
F
|
12-T1 (gonad)
|
5
|
5.6
|
2.0
|
27
|
7
|
6
|
Yes
|
| | |
12-T2 (kidney)
|
7
|
5.5
|
2.2
|
0
|
0
|
6
|
-¶
|
|
13§
|
F
|
13-T1 (spleen)
|
9
|
7.2
|
3.0
|
2.5
|
0
|
7
|
Yes
|
| | |
13-T2 (liver)
|
9
|
8.0
|
1.8
|
0
|
10
|
7
|
Yes
|
| | |
13-T3 (kidney)
|
9
|
7.7
|
2.1
|
10
|
7
|
7
|
Yes
|
- *Mode (most frequent value), mean, and standard deviation (SD) of diploid metaphase cells.
- †Tetraploid cells were identified as those containing four copies of all macrochromosomes and sex chromosomes, and an estimated number of microchromosomes present in twice the normal amount. Aneuploid cells were identified as those containing an abnormal copy number (less than or greater than two) of one or more macrochromosomes.
- ‡Mega-telomere signals were counted in tumor metaphase cells using the telomere-PNA probe, and the mode was calculated for each tumor.
- §Birds from infection strategy 2 (see Methods section for details).
- ¶Tumor 12-T2 lacked a W chromosome (as verified by the W BAC probe, see Table 1).
