Figure 3

Mapping of MDV integrations in chicken chromosomes. An iterative process of mapping by multicolor FISH was used to identify the involved chromosomes. Images shown are from Bird 13, which had nine integration sites (4p, 6p, 9q, 9q, 12, 12, 23, 23, and Wp) for all three tumors. Cells shown in A, B, and C are from tumor 13-T1, which was used to begin mapping. Once the positive sites were identified for 13-T1, they were subsequently tested on 13-T2 (D and E) and 13-T3 (F). Multiple chromosomal BAC probes were tested for possible integration in panels A-C. The MDV positive chromosomes are indicated by superscript. Chromosomal BAC probes are labeled either red (Spectrum Red) or yellow (Spectrum Orange) and in all panels MDV DNA is labeled green (FITC), see Methods. Scale bar, 5 μm