Figure 2
Localization of MDV integrations at the telomeres of chicken chromosomes. Panels A and B show representative results of the MDV integrations (red) observed at the telomeres (green) of the chicken genome of MDV-induced lymphomas. Panel C shows representative chromosomes with MDV integrations (green) to illustrate that integrations were observed in all size-categories of chromosomes (both macro-and microchromosomes) and there was no preference for p or q arm telomere. Panels A and B also illustrate the telomeric array profile observed in the tumors from 15I5 X 71, the heterozygous cross used in this study. For each image, the telomeric signals were adjusted consistently by adjusting GGA 1 interstitial signals so that they were still visible, while not over-saturating the mega-telomere arrays. Chromosomes are blue (DAPI), the telomeres are green (FITC) and the MDV DNA is red (rhodamine, panels A and B) or green (FITC, panel C), see Methods. Scale bar, 5 μm