Figure 5

'Focal' areas of HSV-1 antigen positive cells and demyelinating lesions co-localize throughout the brains of SJL/J, A/J, and PL/J mice. Serial IHC sections counterstained with LFB-CFV were examined for 'focal' areas of viral antigen positive cells and CNS demyelination in the BST and CB. 'Focal' areas and demyelinating lesions co-localize throughout the brains of 10-12 week ♀ SJL/J, A/J, and PL/J mice infected with HSV-1 via the oral mucosa. Initially, island of demyelination are identified within or adjacent to 'focal' areas of viral antigen. Representative sections from the CB of PL/J mice identify these areas within (white arrow) or adjacent to (black arrow) 'focal' areas of viral antigen (a) 8 days PI (x200) and (b) 9 days PI (x200). As the 'focal' areas of viral antigen are cleared, the demyelinating lesions increase in size. Representative sections from the CB of SJL/J mice identify areas of demyelination (arrows) adjacent to 'focal' areas of viral antigen (c) 14 days PI (x200) and (d) 15 days PI (x200). Although individual antigen positive cells can remain in the demyelinating lesions for a period of time (e) antigen positive cell (arrow) in the CB of PL/J mouse, 18 days PI (x200), 'focal' areas of viral antigen are eventually cleared (f) demyelinating lesions in the CB of PL/J mouse, 20 days PI (x200).