Figure 7

Competitive inhibition of ATP binding by maribavir. Protein kinase assays were performed containing increasing concentrations of ATP (2 μM-20 μM) in the absence of maribavir (0.0 μM on the graph) and repeated for increasing maribavir concentrations (0.01 μM, 0.015 μM, 0.2 μM or 0.25 μM). The inverse of the velocity of autophosphorylation was plotted against the corresponding inverse of the [ATP] and the Ki value for maribavir determined using standard enzyme kinetic equations for competitive inhibition.